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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH RIA

Human Factor IX is a glycoprotein which is synthesized in the liver (1–3). The domain structure of factor IX is similar to that of the other vitamin K dependent coagulation factors. The NH2-terminal region contains 12 g-carboxyglutamic acid (gla) residues which facilitate the calcium dependent binding of factor IX to negatively charged phospholipid surfaces. Two domains which are homologous to epidermal growth factor (EGF) span the region between the NH2-terminal gla domain and the activation peptide (Ala-146 to Arg-180).
Factor IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage at site A yields the intermediate IXa which is subsequently converted to the fully active form IXab by cleavage at site B. The NH2-terminal light chain (GLA and EGF domains) remains covalently attached to the COOH-terminal heavy chain by a disulfide bond. The serine protease catalytic triad (Ser-365, His 221, Asp-269) is located in the heavy chain. Factor IXab is the catalytic component of the “intrinsic factor Xase complex” (factor VIIIa/IXa/Ca2+/phospholipid) which proteolytically activates factor X to factor Xa.
Catalog #	F0017
Applications	Suitable for use in RIA, Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA:
	Coating: 5-10ug/ml
	Secondary: 100-1000ug/ml
	Immunohistochemistry: 100ng/ml
	Optimal dilutions to be determined by the researcher.
Extinction Coefficient (1%, 1cm, 280nm)	14.0
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8.H.1
Host	Mouse
Source	Human
Concentration	~10mg/ml
Form	Supplied as a liquid in 50% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Human Factor IX.
Specificity	Recognizes human Factor IX, human Factor IXa and heavy chain of human Factor IX and IXa.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.