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You are here:Home » Antibodies » Antibodies-Coagulation Factors » Anti -Factor IX

Anti -Factor IX


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Coagulation factor IX is a single chain polypeptide with a molecular mass of 57kD. Normal concentrations of factor IX in human plasma is app. 3ug/ml (1). Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.
Catalog #F0017-04
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:2000
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 10% fetal calf serum.
HybridomaX63-Ag8.653. Female Balb/c mice immunized by intraperitoneal injections.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6F98
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenFactor IX isolated from human plasma.
SpecificityRecognizes native human factor IX. Epitope specificity differs from that of clone 6F97 as determined by inhibition ELISA. Strong reaction is seen in ELISA with native factor IX directly coated onto the microtiter plate. In sandwich ELISA it can be used in combination with a polyclonal antibody against factor IX. In Western Blot after SDS-PAGE reacts with factor IX in reduced as well as in non-reduced form.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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