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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Coagulation factor IX is a single chain polypeptide with a molecular mass of 57kD. Normal concentrations of factor IX in human plasma is app. 3ug/ml (1). Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.
Catalog #	F0017-04
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:2000
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 10% fetal calf serum.
Hybridoma	X63-Ag8.653. Female Balb/c mice immunized by intraperitoneal injections.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F98
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Factor IX isolated from human plasma.
Specificity	Recognizes native human factor IX. Epitope specificity differs from that of clone 6F97 as determined by inhibition ELISA. Strong reaction is seen in ELISA with native factor IX directly coated onto the microtiter plate. In sandwich ELISA it can be used in combination with a polyclonal antibody against factor IX. In Western Blot after SDS-PAGE reacts with factor IX in reduced as well as in non-reduced form.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.