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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E

Factor IX (F.IX, Christmas Factor) is a vitamin K-dependent
glycoprotein produced in the liver. Plasma concentration of F.IX is normally around 5ug/ml (87nM) in plasma. The biological
importance of F.IX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in F.IX function. In its zymogen form F.IX is a single chain molecule of 55kD. It contains two EGF-like domains and an amino-terminal domain containing 12 y-carboxy-glutamic acid (Gla) residues. These Gla residues allow F.IX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146- Ala147 and between Arg180-Val181. The terminal activated product in either case is F.IXaB, a two-chain enzyme consisting of a heavy chain (28kD), a light chain (18kD) and an activation peptide product of 11kD. F.IX can also be cleaved into inactive products by thrombin and by elastase. The activity of F.IXaB in plasma is inhibited by antithrombin and this inhibition is accelerated approximately 1000-fold in the presence of optimal concentrations of heparin.
Catalog #	F0017-07
Applications	Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Extinction Coefficient	E1% 280 = 13.4
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~2mg/ml
Form	Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human Factor IX purified from plasma.
Specificity	Recognizes human Factor IX as analyzed by immunoelectrophoresis and ELISA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.