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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor X (HRP)

Anti -Factor X (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E
Factor X (F.X) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of F.X in plasma is ~10ug/ml (~170nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59kD. The light chain (17kD) of F.X contains a calcium-binding domain consisting of one hydroxyaspartic acid and 11 gamma carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of F.X (42kD) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of F.X to the active enzyme (F.Xa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The F.Xa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of F.Xa in plasma is inhibited by antithrombin (ATIII), alpha1-antitrypsin, alpha2-macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated ~1000-fold by heparin.
Catalog #F0018-04
ApplicationsSuitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl A403/A280) 0.48
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in a buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with Horseradish Peroxidase (HRP).
PurityPurified
ImmunogenHuman Factor X purified from plasma.
SpecificityRecognizes human Factor X.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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