Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E Gel

Factor X (F.X) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of F.X in plasma is ~10ug/ml (~170nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59kD. The light chain (17kD) of F.X contains a calcium-binding domain consisting of one hydroxyaspartic acid and 11 gamma carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of F.X (42kD) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of F.X to the active enzyme (F.Xa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The F.Xa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of F.Xa in plasma is inhibited by antithrombin (ATIII), alpha1-antitrypsin, alpha2-macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated ~1000-fold by heparin.
Catalog #	F0018-04
Applications	Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl A403/A280)	0.48
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~2mg/ml
Form	Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with Horseradish Peroxidase (HRP).
Purity	Purified
Immunogen	Human Factor X purified from plasma.
Specificity	Recognizes human Factor X.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.