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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified E B RIA

Bovine Factor X is a vitamin K-dependent protein zymogen which is synthesized in the liver and circulates in plasma as a two chain molecule linked by a disulfide bond (1,2). Prior to secretion into plasma, post-translational modifications produce 11 gamma-carboxyglutamic acid (gla) residues and a single b-hydroxyaspartic acid residue, which are located within the NH2-terminal light chain. The light chain also contains two epidermal growth factor (EGF) homology domains. The COOH-terminal heavy chain of factor X contains most of the carbohydrate moieties, as well as the latent serine protease domain. The activation of factor X is catalyzed by either the intrinsic factor X-ase complex (factor IXa, factor VIIIa, cellular surface and calcium ions) or the extrinsic factor X-ase complex (factor VIIa, tissue factor, cellular surface and calcium ions).
Activation of bovine factor X by either complex results in cleavage at Arg52-Ile53 of the COOH-terminal heavy chain and subsequent release of a 52 amino acid activation glycopeptide. Factor Xa then serves as the enzyme component of the prothrombinase complex which is responsible for the rapid conversion of prothrombin to thrombin. The gla residues enable factor X/Xa to bind phospholipid (i.e. cell surfaces) in a calcium dependent manner; a requirement for assembly of the prothrombinase complex. The first EGF homology domain contains a Ca+2 binding site which acts as a hinge to fold the EGF and GLA domains towards each other (12). This region of the molecule is involved in the recognition of cellular binding domains.
Catalog #	F0018-05
Application	Suitable for RIA, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8.H.5
Host	Mouse
Source	Bovine
Concentration	~5mg/ml
Form	Supplied as a liquid in dH2O, 50% glycerol.
Purity	Purified, >95% by SDS-PAGE
Immunogen	Purified bovine Factor X. MW of Antigen: 150kD
Specificity	Recognizes heavy chain of bovine Factor X, Xa, BEGRck Factor Xa. Does not recognize murine X. Species Crossreactivity: human, rabbit, sheep, porcine and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.