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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E

Factor X (F.X, Stuart Factor) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of F.X in plasma is ~10ug/ml (~170nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59kD. The light chain (17kD) of F.X contains a calcium-binding domain consisting of one hydroxy-aspartic acid and 11 gamma- carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of F.X (42kD) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of F.X to the active enzyme (F.Xa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The F.Xa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of F.Xa in plasma is inhibited by antithrombin (ATIII), alpha1-antitrypsin, alpha2-macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated approximately 1000-fold by heparin.
Catalog #	F0018-08
Applications	Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~2mg/ml
Form	Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human Factor X purified from plasma.
Specificity	Recognizes human Factor X.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.