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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor X

Anti -Factor X

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified E
Factor X (F.X, Stuart Factor) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of F.X in plasma is ~10ug/ml (~170 nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59 kD. The light chain (17 kD) of F.X contains a calcium-binding domain consisting of one hydroxy- aspartic acid and 11 -carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of F.X (42kD) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of F.X to the active enzyme (F.Xa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The F.Xa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of F.Xa in plasma is inhibited by antithrombin (ATIII), alpha-1antitrypsin, alpha-2macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated approximately 1000-fold by heparin1-3.
Catalog #F0018-08A
ApplicationSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Extinction CoefficientE1% 280 = 13.4
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~10mg/ml
FormSupplied in 10mM HEPES, pH 7.4, 150mM sodium chloride, 50% glycerol. No preservative added.
PurityPurified by caprylic acid and ammonium sulfate precipitations, and solid-phase adsorption to remove unwanted crossreactivity.
ImmunogenHuman Factor X purified from plasma.
SpecificityRecognizes human Factor X. Species Crossreactivity: rabbit and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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