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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor XI

Anti -Factor XI

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified E N
Factor XI (F.XI) is a coagulation protein produced in the liver that circulates in plasma at ~5ug/ml (30nM). The mass of F.XI is 160kD as determined by SDS-PAGE under non-reducing conditions and 80kD upon reduction. F.XI consists of two identical 80kD subunits linked by disulphide bonds. Each subunit consists of a tandem repeat of four apple domains followed by a serine protease catalytic domain. Cleavage of F.XI by activated factor XII or thrombin converts each subunit into a two-chain form and generates two active sites per F.XIa molecule. The mass of F.XIa is 160kD unreduced, but upon reduction F.XIa migrates as a heavy chain of 50kD and a light chain of 30kD. The catalytic site of F.XIa resides in the light chain. In plasma, F.XI or F.XIa circulates in non-covalent 1:1 complex with high molecular weight kininogen, which acts as a cofactor in the activation of F.XI by activated factor XII. The activity of F.XIa is regulated by platelets and by several proteinase inhibitors including, in order of decreasing importance, C1-inhibitor, alpha-2antiplasmin, alpha-1antitrypsin and antithrombin. Heparin has relatively little effect on the rate of inhibition of F.XIa by antithrombin. The only known natural substrate for activated F.XI (F.XIa) is factor IX (Christmas factor) and the only cofactor required for this reaction is ionized calcium (1-3).
Catalog #F0018-53A
ApplicationsSuitable for use in ELISA, Immunoelectrophoresis and Neutralization. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~5mg/ml
FormSupplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
PurityPurified
ImmunogenFactor XI (F.XI) purified from human plasma.
SpecificityRecognizes human Factor XI.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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