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Anti -Factor XII Light Chain (Hageman Factor)
Anti -Factor XII Light Chain (Hageman Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E N |
|
| Human Factor XII (XII) (Hageman Factor) is a single chain (78kD) glycoprotein zymogen that circulates in plasma at a concentration of 40 mg/ml. Reciprical activation of XII to the active serine protease factor XIIa (XIIa) by kallikrein is central to initiation of the intrinsic coagulation pathway. Surface bound a-XIIa in turn activates factor XI to XIa. A single cleavage by kallikrein at R353-Val354 of XII yields a-XIIa, a 2 chain protease (80kD) held together by disulfide bonds. The COOH-terminal light chain (28kD) contains the catalytic triad (His-40, Asp-89, Ser-191), while the NH2-terminal heavy chain (52kD) conatins the anionic surface binding portion of the molecule. A secondary cleavage of a-XIIa by kallikrein outside the disulfide bond yields b-XIIa (XIIf, BHFa, HFf, hageman factor fragments) (28kD), which no longer binds anionic surfaces. b-XIIa can activate prekallikrein, but has little procoagulant activity. | | | Inhibitors of XIIa include C1-INH, a2-antiplasmin, a2-macroglobulin and antithrombin III. At physiological concentrations, the relative effectiveness of these inhibitors is 91: 4.5:3:1.5, respectively. The ratio of C1-INH to XII has been implicated in the “cold activation” of factor VII and the conversion of prorenin to renin on storage of plasma. | | | Catalog # | F0019-04 | | Applications | Suitable for use in ELISA, Inhibition and Coagulation assays. Other applications not tested. | | Recommended Dilutions | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 1.B.694 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. No preservative added. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified human Factor XII. | | Specificity | Recognizes the light chain of human Factor XII and at 100nM inhibits 50% of Factor XII activity. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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