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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor XII (Hageman Factor) (HRP)

Anti -Factor XII (Hageman Factor) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified E Gel
Factor XII (F.XII, Hageman factor) is a 76kD, single chain
glycoprotein produced in the liver. In plasma, F.XII circulates as a protease zymogen at a concentration of approximately 30ug/ml (400nM). Upon vascular injury F.XII binds to negatively charged extravascular surfaces such as cartilage and skin, which facilitate activation of the zymogen to the active serine protease. Cleavage of F.XII by kallikrein after residue Arg353 produces the enzyme alphaF.XIIa, consisting of a 28kD light chain containing the protease domain, and a 52kD heavy chain containing the anionic surface-binding domain. Substrates for surface bound F.XIIa include the zymogens prekallikrein(PK) and factor XI(F.XI) as well as the procofactor high-molecular weight kininogen (HK). The activation of these substrates results in positive feedback activation of F.XII. Further cleavage of alphaF.XIIa by kallikrein produces the 28kD fragment betaF.XIIa (Hageman factor fragment). BetaF.XIIa has reduced procoagulant activity as it lacks the anionic surface-binding domain, but is capable of fluid-phase activation of PK, factor VII and complement C1. The activity of F.XIIa in plasma is regulated predominantly by C1-Inhibitor, with relatively minor contributions by alpha2antiplasmin, alpha2macroglobulin
and antithrombin, even in the presence of therapeutic levels of
heparin(1-3).
Catalog #F0019-06B
ApplicationsSuitable for use as detecting antibody in ELISA, Immunoelectrophoresis and other solid phase immunoassays for Factor XII and its activation products. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl, A403/A280)0.43
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
PurityPurified
ImmunogenActivated human Factor XII (F.XII) purified from plasma.
SpecificityRecognizes human Factor XII at ~76kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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