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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor XIII

Anti -Factor XIII

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E
Factor XIII (F.XIII, fibrin stabilizing factor) is the proenzyme form of a transamidase that is essential for normal haemostasis and
fibrinolysis, wound healing, female fertility and foetal development. Extracellular F.XIII consists of A subunits (83kD each) which contain the enzyme moiety, and B subunits (76kD each) which act as a carrier protein for the A subunit in circulation. Both subunits are produced under separate genetic control. In plasma, F.XIII exists as a non-covalent tetrameric complex (320kD) of two A-subunits and two B-subunits (A2B2). The concentration of F.XIII tetramer in plasma
is ~25ug/ml (~80nM). An intracellular form of F.XIII is found in platelets, megakaryocytes and monocytes. This form of F.XIII
presents as a dimer of two A-subunits only and has a molecular
weight of 160kD. The importance of these intracellular stores is demonstrated by the observation that platelets can contribute up to half of the F.XIII activity in platelet rich plasma. The activation of F.XIII involves several steps. Thrombin cleaves after Arg37 of each A-subunit in the A2B2 tetramer, releasing a 4.5kD activation peptide. Additional conformational changes induced by the binding of calcium, and by dissociation of the B-subunits from the A-subunit dimer are required to obtain full enzyme activity. F.XIIIa is a cysteine protease that catalyses the formation of -glutamyl-e-lysyl bonds between the and chains of polymerised fibrin molecules. Other proteins found crosslinked into fibrin clots by F.XIIIa include fibrinogen, a-2antiplasmin, fibronectin, vitronectin and von Willebrand factor
Catalog #F0019-45
ApplicationsSuitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Extinction Coefficient (E1% 280) 13.4
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~10mg/ml
FormSupplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
PurityPurified
ImmunogenHuman Factor XIII (A2B2) purified from plasma
SpecificityRecognizes human Factor XIII as demonstrated by immunoelectrophoresis and ELISA. Species Crossreactivity: rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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