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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor XIII subunit A

Anti -Factor XIII subunit A

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E
Factor XIII (F.XIII, fibrin stabilizing factor) is the proenzyme form of a transamidase that is essential for normal haemostasis and fibrinolysis, wound healing, female fertility and foetal development. Extracellular F.XIII consists of A subunits (83kD each) which contain the enzyme moiety, and B subunits (76 kD each) which act as a carrier protein for the A subunit in circulation. Both subunits are produced under separate genetic control. In plasma, F.XIII exists as a non-covalent tetrameric complex (320kD) of two A-subunits and two B-subunits (A2B2). The concentration of F.XIII tetramer in plasma is ~25ug/ml (~80nM). An intracellular form of F.XIII is found in platelets, megakaryocytes and monocytes. This form of F.XIII presents as a dimer of two A-subunits only and has a molecular weight of 160 kD.The importance of these intracellular stores is demonstrated by the observation that platelets can contribute up to half of the F.XIII activity in platelet rich plasma. The activation of F.XIII involves several steps. Thrombin cleaves after Arg37 of each A- subunit in the A2B2 tetramer, releasing a 4.5 kDa activation peptide. Additional conformational changes induced by the binding of calcium, and by dissociation of the B-subunits from the A-subunit dimer are required to obtain full enzyme activity. F.XIIIa is a cysteine protease that catalyses the formation of -glutamyl- -lysyl bonds between the and alpha chains of polymerised fibrin molecules. Other proteins found crosslinked into fibrin clots by F.XIIIa include fibrinogen, alpha 2antiplasmin, fibronectin, vitronectin and von Willebrand factor
Catalog #F0019-46A
ApplicationsSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol. No preservatives are added.
PurityPurified by affinity chromatography.
ImmunogenFactor XIII subunit A purified from human plasma.
SpecificityRecognizes human Factor XIII as determined by immunoelectrophoresis and ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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