| Epitope localizes in the C-terminal (aa 853-1052) of human FAK protein. FAK is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Excellent for multiple biochemical applications. |
|
| Catalog # | F0019-54 |
| Applications Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blotting. Other applications have not been tested. |
| Recommended Dilution | Immunoprecipitation: 10ug/mg protein lysate. Use Protein A. |
| Western Blotting: 5-10ug/ml for 2hrs at RT |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | HUVEC or IMR-5 cells . |
| Cellular Localization | Cytoplasmic and focal contacts |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | 1mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide, cat#F0019-54X. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Aa 853-1052 from the C-terminus of human FAK. |
| Specificity | Recognizes a 125kD protein, which is identified as FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) of human, mouse, rat, canine and frog. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
