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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) (BSA & Azide Free)

Anti -FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Epitope localizes in the C-terminal (aa 853-1052) of human FAK protein. FAK is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
Catalog #F0019-54X
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 5-10ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlHUVEC, SW156 and IMR-5 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide, F0019-54.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK. Cellular Localization: Cytoplasmic and focal contacts.
SpecificityRecognizes human FAK. Species crossreactivity: Mouse, rat, canine and frog.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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