Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Anti -FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IF
FAK is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine- phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
Catalog #F0019-55
ApplicationsSuitable for use in Immunoprecipitation and Immunofluorescence. Not recommended for Western Blot.
Other applications have not been tested.
Recommended DilutionImmunofluorescence: 10ug/ml
Immunoprecipitation/Western Blot: 4ug immunoprecipitates FAK from 500ug of a mouse 3T3/A31 cell RIPA lysate. The immunoprecipitate was resolved by electrophoresis, transferred to nitrocellulose and probed with F0019-56 (2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.43
HostMouse
SourceChicken
Concentration~0.5mg/ml
FormSupplied as liquid in PBS, pH 7.2, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenMixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing p125 FAK.
SpecificityRecognizes (and is specific for) chicken p125 FAK. Antibody has different affinity for modified forms of FAK and alternate splicing transcripts of FAK. Species Crossreactivity: human, rodent and avian.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links