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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Anti -FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb 2 adapter protein and c Src PTK with FAK in vivo, and also results in activation of the ERK 2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb 2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb 2 SH2 domain binds directly to tyrosine phosphorylated FAK, and the binding site has been identified as Tyr 925 by site directed mutagenesis.
Catalog #F0019-55A7
Cellular Localization Cytoplasmic and focal contacts.
Positive Control HUVEC, SW156 or IMR-5 cells.
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
Form10mM PBS, pH7.4, 0.2%BSA
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant fragment, corresponding to amino acids 853-1052 of Human FAK.
SpecificitySpecies Crossreactivity: Crossreacts with canine, Human, Mouse, Rat and Xenopus laevis.Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesFocal Adhesion Kinase, FADK 1, pp125FAK, Protein Tyrosine Kinase 2


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