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Anti -FAK, phosphorylated (Tyr397) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Anti -FAK, phosphorylated (Tyr397) (Focal Adhesion Associated Protein Tyrosine Kinase,
BC3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| FAK is a cytoplaslmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125aa sequence at the C terminus called the “Focal Adhesion Targeting” sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr 576 and Tyr 577) in the kinase domain activation loop. | | | Catalog # | F0019-55J6 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500-1:1000 | | Optimal dilutions to be determined by the researcher. | | Titer | ELISA (immunogen): 1:16,000 | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E). | | Specificity | Recognizes endogenous levels of human FAK at ~125kD, only when phosphorylated at tyrosine 397. Species crossreactivity: rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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