| Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that was discovered as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Phosphorylation of tyrosine 407 is activated by TGFb and Epithelial Mesenchyme Transition (EMT). Unlike other sites on FAK, tyrosine 407 is EGFR independent. |
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| Catalog # | F0019-55K |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: A 1:1000 detected FAK phosphorylated on residue Tyr407 in lysate from FAK transfected CEF cells grown on fibronectin. |
| Optimal dilution to be determined by researcher. |
| Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Suplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/mL BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to amino acid region encompassing the human, mouse, rat, and chicken phospho-FAK (Tyr407). Species Sequence Homology: mouse, rat and chicken. |
| Specificity | Recognizes human phospho-FAK (Tyr407), Mr 125kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
