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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK, phosphorylated (Tyr576) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Anti -FAK, phosphorylated (Tyr576) (Focal Adhesion Associated Protein Tyrosine Kinase,
BC3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The phosphorylation by Src of tyrosines 576 and 577 in the activation loop results in maximal activity of FAK. However, the two sites appear to be differentially regulated by stimuli.
Catalog #F0019-56B1
ApplicationsSuitable for use in ELISA, Western Blot and previous lots have been used in Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlChicken embryo fibroblasts (CEFs) expressing FAK protein and plated on fibronectin and treated with vanadate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human FAK that contains tyrosine 576.
SpecificityRecognizes human FAK, phosphorylated (Tyr576). Species sequence homology: mouse, rat, chicken and frog.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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