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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK, phosphorylated (Ser732) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Anti -FAK, phosphorylated (Ser732) (Focal Adhesion Associated Protein Tyrosine Kinase,
BC3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that plays a key role in signaling by growth factors, extracellular matrix and stress signals. Indeed, FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The serine/threonine kinase cyclin-dependent kinase-5 (Cdk5)-an important regulator in brain development-phosphorylates serine 732 of FAK, possibly regulating a centrosome-associated microtubule structure to promote nuclear translocation, a critical step in neuronal migration. This phosphorylation does not have a direct impact on the kinase activity of FAK, but appears to prevent the accumulation of FAK at the centrosome.
Catalog #F0019-56G
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlPC12 cells treated with nocozadole; cdk5 +/- and cdk5 -/- mouse brain lysates.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human FAK that contains serine 732. Species sequence homology: mouse, rat and chicken.
SpecificityRecognizes mouse and rat FAK when phosphorylated at Ser732. Species sequence homology: human (100%), chicken (100%) and frog (90%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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