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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK (Focal Adhesion Kinase 1, FADK 1, Focal Adhesion Kinase-related Nonkinase, FRNK, Protein Phosphatase 1 Regulatory Subunit 71, PPP1R71, Protein-tyrosine Kinase 2, p125FAK, pp125FAK, PTK2, FAK1)

Anti -FAK (Focal Adhesion Kinase 1, FADK 1, Focal Adhesion Kinase-related Nonkinase, FRNK,
Protein Phosphatase 1 Regulatory Subunit 71, PPP1R71, Protein-tyrosine Kinase 2, p125FAK,
pp125FAK, PTK2, FAK1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC IF
Focal adhesion kinase was identified as a 125kD substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced aa sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein coupled receptors.
Catalog #F0019-57D
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunoprecipitation: 5-20ug/ml
Immunocytochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Numbern/a
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to human FAK.
SpecificityRecognizes human FAK at ~125kD. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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