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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -FAK, phosphorylated (Tyr576/577)

Anti -FAK, phosphorylated (Tyr576/577)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases (2,3), PI3K and PLCgamma (4,5). The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the C-terminal region of FAK (6,7). Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6).
Catalog #F0019-58T
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr576/577 of human FAK.
SpecificityDetects endogenous levels of Tyr576/577 phosphorylated FAK.Product Type: Pab
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr576/577 of human FAK.
SpecificityDetects endogenous levels of Tyr576/577 phosphorylated FAK. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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