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You are here:Home » Cloning » Cloning-Primers » Fas Ligand (FASL, TNFSF6), Human, Mouse, Primer Pair

Fas Ligand (FASL, TNFSF6), Human, Mouse, Primer Pair

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Specifications

Human/mouse Fas Ligand/TNFSF6 specific PCR primers for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Catalog #F0019-66P
ComponentsPrimer Pair: Lyophilized. Each vial contains 375 pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25°C; 0.1mM EDTA, pH 8.0 @ 25°C).
Positive Control 5 Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes.
GenBank Accession NumberX89102 (human) and U06948 (mouse)
Product SizescDNA: 239 base pairs (bp).
Genomic DNA: Sequence not available. No products observed.
Positive Control 5: 275 bp
Alternate splice variants: None reported.
Suggested Reaction ConditionsFor each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment.
Thermocycling ConditionsA typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sutoclaved ddH2O or 0.1X TE buffer. Aliquot and store at -20°C. Stable for only one thaw. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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