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You are here:Home » Antibodies » Abs to Fatty Acid Binding Protein » Anti -Fatty Acid Binding Protein, Heart, Adipocyte (FABP)

Anti -Fatty Acid Binding Protein, Heart, Adipocyte (FABP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD). FABP’s have a widespread tissue distribution. FABPs are quite abundant (3-5% of total cellular protein). At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine. Several different isoforms of FABP have been identified. These isoforms are generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain, etc. For example, protein designated as H-FABP indicates that it is heart type). Expression of these isoforms is not exclusive. More than one isoform can be found in a given cell or tissue. Three main types of FABPs that were initially discovered in the heart (FABP-H), liver (FABP-L), and intestine (FABP-I) are not exclusive to these tissues and show considerable differences at the amino acid level (~30% identity). Other FABPs detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs.
Catalog #F0019-77
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000.
ELISA: 1:1,000-1:10,000. Coat with 5-10ug F0019-77E.
Optimal dilutions to be determined by the researcher.
Control PeptidesF0019-77E: Fatty Acid Binding Protein, Heart, Adipocyte, Human (FABP), Control Peptide
F0019-77M: Fatty Acid Binding Protein, Human, Heart, Adipocyte, Human (FABP) Western Blot Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1% sodium azide, 40% glycerol.
PuritySerum
Immunogen12aa synthetic peptide corresponding to the C-terminus of human FABP (KLH).
SpecificityRecognizes Fatty Acid Binding Protein (FABP), Heart, Adipocyte. Isoform Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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