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You are here:Home » Antibodies » Antibodies-Fatty Acid Binding Protein » Anti -Fatty Acid Binding Protein, Heart (FABP)

Anti -Fatty Acid Binding Protein, Heart (FABP)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD) and proteins that has widespread tissue distribution. FABPs are quite abundant (3-5% of total cellular protein). At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine. Several different isoform of FABP have been identified and generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain etc; protein designated as H-FABP indicates that it is heart type). However, expression of these isoform is not exclusive and more than one isoform can be found in aH given cell or tissue. Three main types of FABPs that were initially discovered in the heart (FABP-), liver (FABP-L), and intestine (FABP-I) are not exclusive these tissues and show considerable differences at the amino acid level (~30% identity). Other FABPs recently detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs. FABPs are also known as mammary derived growth inhibitor (MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2 homolog, P2 adipocyte protein, 422 protein (P15). Human FABP-H or adipocyte is 132-aa protein (chromosome 2p11) Rat and mouse adipocyte-FABPs are 133 aa single polypeptide chains.
Catalog #F0019-77G
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000. Use +ve as control (refer to Catalog F0019-77M). Load ~10ul/lane.
ELISA: 1:10,000-1:50,000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3H429
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified human heart FABP (> 98%).
SpecificityRecognizes human heart FABP. Isoform crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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