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You are here:Home » Molecular Biology » MB-Fatty Acid Binding Protein » Fatty Acid Binding Protein 4, Recombinant, Human (FABP4, A-FABP)

Fatty Acid Binding Protein 4, Recombinant, Human (FABP4, A-FABP)

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Specifications

Adipocyte fatty acid binding protein FABP4 is a 15kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. FABP4 is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells. In mice, targeted mutations in FABP4 provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from FABP4-deficient mice also have reduced efficiency of ipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated FABP4 expression in macrophages upon differentiation and activation. In these cells, FABP4 modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific FABP4 deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for FABP4 in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages.
Catalog #F0019-82D
DescriptionRecombinant Human Fatty Acid Binding Protein 4 is a monomeric ,non-glycosylated, polypeptide chain containing 132 amino acids and having a molecular mass of 14,700 Daltons.
SpecificityThe amino acid sequence of the recombinant human FABP4 is 100% homologous to the amino acid sequence of the human FABP4.
ApplicationsWestern blotting, ELISA
Reconstitution0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.
StorageStore lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid freeze-thaw cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Molecular Weight14.7kD
SourceE. coli
Purity90% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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