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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E IH

Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However. Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells.
Catalog #	F0019-96
Comments	Can be used as an effective marker for Reed Sternberg cells.
Applications	Immunohistology (Formalin/paraffin): (Use Ab at 1:100-1:200 for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control	Hodgkin’s lymphoma or spleen
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5G112
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid with 0.09% sodium azide.
Purity	Culture supernatant.
Immunogen	Fascin purified from HeLa cells.
Specificity	Recognizes Fascin. Species Crossreactivity: Human and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.