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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Fascin (Singed-like Protein, 55kD Actin-bundling Protein, p55, SNL, HSN, FAN1, FSCN1)

Anti -Fascin (Singed-like Protein, 55kD Actin-bundling Protein, p55, SNL, HSN, FAN1,


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Clone Host Grade Applications
Monoclonal Mouse Purified IH
Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene. Localized in microspikes and stress fibers of cultured cells, it is hypothesize to be involved in the formation of microfilament bundles. Fascin expression is predominantly located in dendritic cells, while negative in regions of lymphoid, myeloid, and plasma cells. However, Reed Sternberg cells in Hodgkin's lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells.
Catalog #F0019-96F
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlHodgkin’s lymphoma, spleen
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoSPM133
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
ImmunogenFascin protein purified from HeLa cells
SpecificityRecognizes Fascin. Species Crossreactivity: human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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