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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -FBG4 (F-box 4)

Anti -FBG4 (F-box 4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase
complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.
Catalog #F0019-98J
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Optimal dilutions to be determined by the researcher.
Positive control Recombinant human full-length FBG4.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa70-85 (LQLARDRSAEGRALYA) of human FBG4 conjugated to KLH at the N-terminus.
SpecificityRecognizes human FBG4 (aa70-85). Sequence homology: mouse, rat (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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