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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -FBG5 (F-box 5)

Anti -FBG5 (F-box 5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.
Catalog #F0019-98K
ApplicationsWestern Blot: Use at 1:500 to 1:1000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
SpecificityReacts with residues 163-175 (KKQVLDLEEEGLW) of the human FBG5 protein. 76% identical to mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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