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F0020-13A Goat Anti-FASN (Fatty Acid Synthase) [KO Validated]

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
P49327
Isotype
IgG
Grade
Affinity Purified
Applications
E WB KO
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

FASN (Fatty Acid SyNthase; also FAS) is a cytosolic member of the short chain dehydrogenase/ reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511aa in length. It contains multiple domains, including a B-ketoacyl synthase domain (aa1-414), an acyl and malonyl transferase domain (aa429-817), an enoyl reductase domain (aa1563-1863), a B-ketoacyl reductase domain (aa1864-2118) and a thioesterase domain (aa2207-2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa10-212, human FASN shares 92% aa identity with mouse FASN.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Knockout: Fatty Acid Synthase/FASN is specifically detected in HEK293 human embryonic kidney parental cell line but is not detectable in Fatty Acid Synthase/FASN knockout HEK293 cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ser10-Cys212 from human Fatty Acid Synthase, expressed in E. coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human FASN.
Conjugates
Pricing
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