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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -FBXO32 (F-box Only Protein 32, Atrogin-1, Muscle Atrophy F-box Protein, MAFbx)

Anti -FBXO32 (F-box Only Protein 32, Atrogin-1, Muscle Atrophy F-box Protein, MAFbx)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Catalog #F0023-86L
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in human and mouse skeletal muscle lysates on ~40kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-NSKTKTQYFHQEK, from FBXO32, at the internal region of the protein (NP_478136.1).
SpecificityRecognizes human FBXO32. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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