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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -FBXO43 (F-box Only Protein 43, Endogenous Meiotic Inhibitor 2, EMI2, FBX43)

Anti -FBXO43 (F-box Only Protein 43, Endogenous Meiotic Inhibitor 2, EMI2, FBX43)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC IF
Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
Catalog #F0024-43A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-PGSAQSKRNLKRL, from rat FBXO43, at C-terminal (NP_001025031.2).
SpecificityRecognizes rat FBXO43. Species sequence homology: Bovine, canine, human, mouse and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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