| F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. |
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| Catalog # | F0024-72A |
| Applications | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | |
| Peptide ELISA Titer: 1:128,000 |
| Western Blot: 0.3-1.5ug/ml; ~48kD band observed in Human Kidney lysates (calculated MW of 48.0kD according to NP_036296.1). A stronger band of unknown identity was also consistently observed at appr. 30kD - both bands were successfully blocked by incubation with the immunising peptide. |
| Immunohistochemistry: 2-3ug/ml In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. |
| Purity | Purified by peptide affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to C-KRGSSFLAGEHPG, from the C Terminus of human FBXW2 (NP_036296.1). |
| Specificity | Recognizes human FBXW2. Species sequence homology: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | F-box and WD repeat domain containing 2, F-box protein Fbw2, Md6, MGC117371, Fwd2, FBW2, F-box and WD-40 domain protein 2, FBXW2 |
