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You are here:Home » Antibodies » Abs to Amyloid » Anti -Fe65

Anti -Fe65

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IC
Amyloid precursor protein (APP) is a cell-surface protein that is cleaved by g-secretase at the transmembrane region into an extracellular amyloid-beta peptide (Ab) and an intracellular tail fragment. This cytoplasmic tail of APP forms a multimeric complex with the nuclear adaptor protein Fe65 and the histone acetyltransferase Tip60. Fe65 is an adaptor protein that bridges APP to certain molecular pathways. The interaction between APP and Fe65 increases the translocation of APP to the cell surface and the subsequent secretion of Ab. Fe65 is thus important in the regulation and trafficking of APP. Low density lipoprotein receptor-related protein (LRP) also mediates APP processing and is involved with Alzheimer's disease pathogenesis by affecting Ab and APP C-terminal fragment generation. A recent study has shown that Fe65 influences the LRP-mediated effect on APP processing by acting as a functional linker between APP and LRP.
Catalog #F0027D
ApplicationsSuitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunocytochemistry: Reported to detect Fe65 in rat primary neurons fixed with 4% paraformaldehyde.
Western Blot: 1:1000-1:10,000 dilution detects Fe65 in homogenates prepared from whole mouse, rat and human Alzheimer’s diseased brains.
Immunoprecipitation: 5ul immunoprecipitated Fe65 from 500ug of rat brain homogenate.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H324
HostMouse
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityAscites
ImmunogenFusion protein corresponding to aa254-293 of mouse Fe65, WW domain.
SpecificityRecognizes mouse Fe65, Mr ~97kD. Species Crossreactivity: rat and human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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