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F0045-01 Goat Anti-FEM1A (Protein Fem-1 Homolog A, FEM1a, FEM1-alpha, Prostaglandin E Receptor 4-associated Protein, EPRAP)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Ca Hu
Accession #
NP_061178.1
Gene ID
55527
Shipping Temp
Blue Ice
Storage Temp
-20°C

Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 1-3ug/ml, observed in human heart lysates on ~73.6kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-GFIPEDLEAFIELH, from human FEM1A, at C-terminal (NP_061178.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human FEM1A. Species sequence homology: Bovine and canine.
References
1. Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P. Prostaglandin E receptor type 4-associated protein interacts directly with NF-kappaB1 and attenuates macrophage activation. J Biol Chem. 2008 Apr 11;283(15):9692-703.
USBio References
No references available
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