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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)

Anti -Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2,
DNase IV)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.
Catalog #F0046
Application(s)Suitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5mg/ml
Optimal dilutions to be determined by the researcher.
HybridomaFusion between BALB/c splenocytes and mouse myeloma (NS-1 cells).
Positive ControlMCF-7 cells, recombinant fusion protein.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4i261
FormSupplied as a liquid in 10mM PBS (pH 7.4).
PurityPurified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
ImmunogenRecombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus-infected Sf-9 cells.
SpecificityReacts with human FEN-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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