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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)

Anti -Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2,
DNase IV)

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Specifications

Clone Host Grade Applications
Polyclonal Mouse Serum B
FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42kD protein that functions as an endonuclease and cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems.
Catalog #F0047
Application(s)Suitable for use in Western Blot. HepG2, HeLa, T24 and HBL100 cell lysates have been tested. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlRecombinant fusion protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySerum
ImmunogenRecombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus infected Sf9 cells.
SpecificityReacts with human FEN-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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