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You are here:Home » Antibodies » Abs to FAM (Family with Sequence Similarity) » Anti -FAM3A (Family with Sequence Similarity 3 Member A)

Anti -FAM3A (Family with Sequence Similarity 3 Member A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
FAM3A, (Family with sequence similarity 3, member A) also known as "2-19" is a member of a short-chain four alpha-helical bundle cytokine family. Little is known about it other than it is expressed in vascular endothelium and pancreatic duct epithelium. It is synthesized as a 230 amino acid (aa) precursor that contains a 33aa signal sequence and a 197aa mature region. It will likely be glycosylated with a molecular weight around 22kD. Mature human FAM3A is ~93% aa identical to mature mouse and rat FAM3A.
Catalog #F2954-15A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml;. The detection limit is ~13ng/well.
Western Blot: 1-2ug/ml; Using a colorimetric detection system, the detection limit is ~25 ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Hybridomamouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human FAM3A (rhFAM3A; aa 34-230: Accession # AAH08912).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H132
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human FAM3A
SpecificityRecognizes human FAM3A at ~22kD. Does not cross react with recombinant human FAM3B, FAM3C or FAM3D. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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