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You are here:Home » Antibodies » Abs to FAM (Family with Sequence Similarity) » Anti -FAM3D (Family with Sequence Similarity 3 Member D, EF-7)

Anti -FAM3D (Family with Sequence Similarity 3 Member D, EF-7)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Family with sequence similarity 3, member D (FAM3D), also known as EF-7, belongs to the novel cytokine-like family whose members are secreted four-helix bundle proteins. Human FAM3D is abundantly expressed in placenta as a 224 amino acid (aa) residue precursor with a 25aa signal sequence. The aa sequence of mature human FAM3D is 74% identical to that of mouse FAM3D.
Catalog #F2954-16D
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant human FAM3D is ~50ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human FAM3D is ~13ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H133
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa39-224 from human FAM3D, expressed in NSO cells (NP_620160).
SpecificityRecognizes human FAM3D. Does not crossreact with recombinant human FAM3A, FAM3B, FAM3C or recombinant mouse FAM3D.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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