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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified E

Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. Natural iron is quite insoluble. Excess iron is harmful. It can catalyze the formation of potentially damaging reactive oxygen species. Cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels. The major pool of body iron (~85%; 40-50mg/kg) is found in circulating hemoglobin and muscle myoglobin. Iron absorption occurs primarily in the intestine (duodenum) and inversely related to body iron reserve. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), iron regulatory proteins (IRPs), etc. play a key role in iron metabolism. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Its three dimensional structure is conserved in all species despite very low sequence identity from bacteria to human. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~ 19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H:L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy.
Catalog #	F4015
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Affinity Constant	~2.0x10e10 L/mole
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8.F.111
Host	Mouse
Source	Human
Concentration	8mg/ml
Form	Supplied as a liquid in PBS, pH 7.5, 0.1% sodium azide.
Purity	Purified by chromatography.
Immunogen	Human liver ferritin
Specificity	Recognizes human liver ferritin
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.