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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IP IH Gel

Catalog #	F4015-12
Applications	Suitable for use in Western Blot, Dot Blot, ELISA, conjugation and Immunohistochemistry. Other applications not tested.
	Recommended Dilution:
	ELISA: 1:5000 to 1:20,000
	Western Blot: 1:500-1:2000
	Immunohistochemistry: 1:200-1:1000
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~10mg/ml
Form	Supplied as a lliquid in PBS, pH 7.2, in 0.01% sodium azide, 40% glycerol.
Purity	Purified by delipidation, defibrinated and salt fractionated and by ion exchange chromatography.
Immunogen	Human spleen ferritin.
Specificity	Recognizes human ferritin. May recognize ferritin from other tissues and species. Immunoelectrophoretic assay resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified ferritin (human spleen).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.