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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IH

Ferritin is the major intracellular iron storage protein in all organisms. It has the shape of a hollow sphere that permits entry of a variable amount of iron for storage as ferric hydroxide phosphate complexes. Mammalian liver and spleen ferritin (relative mass about 450,000) consists of 24 subunits of 2 species, the heavy subunit (relative mass = 21,000) and the light subunit (relative mass = 19,000).
Catalog #	F4015-13
Application	Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:20,000- 1:100,000
	Western Blot: 1:2000-1:10,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~10mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Purity	Puri ed by delipidation, salt fractionation and ion exchange chromatography.
Immunogen	Human spleen ferritin.
Specificity	Recognizes human ferritin. Assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified ferritin from human spleen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.