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You are here:Home » Antibodies » Abs to Serum Proteins » Anti -Ferritin, Spleen (HRP)

Anti -Ferritin, Spleen (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH
Ferritin is the major intracellular iron storage protein in all organisms. It has the shape of a hollow sphere that permits entry of a variable amount of iron for storage as ferric hydroxide phosphate complexes. Mammalian liver and spleen ferritin (relative mass about 450,000) consists of 24 subunits of 2 species, the heavy subunit (relative mass = 21,000) and the light subunit (relative mass = 19,000).
Catalog #F4015-14
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:2000
ELISA: 1:2000-1:10,000
Capture ELISA: 1:12,000-1:60,000. Assayed against 1ug of Ferritin (human spleen) using ABTS (A0030) as a substrate for 30 minutes at RT.
Immunohistochemistry: 1:200-1:1000
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).
PurityPurified by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenHuman spleen ferritin
SpecificityRecognizes human ferritin. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified ferritin (human spleen).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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