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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Elemental iron is required for a variety of normal cellular functions and is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains, where 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3 and FTH subunit (rich in human heart) is located on chromosome 11. Ferritin is capable of storing up to 4,500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner and is also influenced by pathophysiological conditions, including inflammation and malignancy. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation and L-chains assist in core formation.
Catalog #	F4015-40
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 0.5-1ug/ml.
	Western Blot: 1:1000-1:5000 using ECL
	Optimal dilutions to be determined by the researcher.
Positive Control for Western Blot	F4015-55: Ferritin H-chain, Human, Western Blot Pos. Control
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS. No BSA or azide added.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to 15aa near the NT of human/mouse/rat FTH, conjugated to KLH. Species Sequence Homology (100%): mouse, rat, bovine, monkey and chicken
Specificity	Recognizes human Ferritin H-chain (FTH). No sequence homology with Ferritin L-chain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.