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You are here:Home » Antibodies » Abs to Serum Proteins » Anti -Ferritin H-chain (FTH)

Anti -Ferritin H-chain (FTH)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Elemental iron is required for a variety of normal cellular functions. It is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains. 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit is coded by a gene in segment 19q13.3. FTH subunit is located on chromosome 11. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy. Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation. L-chains assist in core formation.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #F4015-45
Recommended DilutionWestern Blot: 1:1000-1:5000 using ECL.
ELISA: 10,000-50,000. Control peptide can be used to coat plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control PeptideF4015-50: Ferritin H-chain, Human, Control Peptide (FTH)
F4015-55: Ferritin H-chain, Human, Western Blot Positive Control (FTH)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide (15aa) near the NT of human/mouse/rat FTH (KLH).
SpecificityRecognizes Human FTH heavy chain. No sequence homology with ferritin L-chain. Sequnce Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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