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Anti -a-Fetoprotein (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (BSA & Azide Free)
Anti -a-Fetoprotein (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein
precursor, FETA, HPAFP) (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH |
|
| AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. | | | Catalog # | F4100-12X | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry: 1:10-1:20 for 30 minutes at RT. No special pretreatment is required for staining of formalin fixed, paraffin embedded tissues. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Fetal liver or hepatocellular carcinoma. | | Source | Ascites | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 0.N.13 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. No preservatives or stabilizing proteins added. Also available with BSA and azide. See F4100-12. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Affinity purified alpha fetoprotein from serum of hepatoma patient. Cellular Localization: Cytoplasmic. | | Specificity | Recognizes human alpha fetoprotein (AFP) at 70kD. Does not react with other onco-fetal antigens or serum albumin. Species Crossreactivity: canine and porcine. Does not react with bovine, feline, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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