Anti -FHL1 (Four and a Half LIM Domains 1, FHL-1, FHL1A, FHL1B, FLH1A, bA535K18.1, KyoT,
LIM Protein SLIMMER, MGC111107, RBP Associated Molecule 14-1, RAM14-1, Skeletal Muscle LIM
Protein 1, SLIM1, SLIM-1, SLIM, XMPMA)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B |
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| FHL1 is a 36kD member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323aa in length. It contains one C4-type Zn-finger region (aa7-31), three distinct LIM domains (aa40-77; 101-158; 162-212) and a bipartite NLS (aa231-246). There is one 32kD splice variant that shows a 50 aa substitution for aa231-323. This creates a fourth LIM domain. There are also alternative start sites aa29-16 upstream of the standard site, a deletion of aa168-296, and an 11 aa substitution for aa81-91. Over aa93-166, human FHL1 is 97aa identical to mouse FHL1. | | | Catalog # | F4149-77E1 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 200ug/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa93-166 from human FHL1 expressed in E. coli (Q13642). | | Specificity | Recognizes human FHL1. Species sequence homology: recombinant human FHL2 and rhFHL3 (1%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -FHL1 (Four and a Half LIM Domains 1, FHL-1, FHL1A, FHL1B, FLH1A, bA535K18.1, KyoT, LIM Protein SLIMMER, MGC111107, RBP Associated Molecule 14-1, RAM14-1, Skeletal Muscle LIM Protein 1, SLIM1, SLIM-1, SLIM, XMPMA) |
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