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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -FHL1 (Four and a Half LIM Domains 1, FHL-1, FHL1A, FHL1B, FLH1A, bA535K18.1, KyoT, LIM Protein SLIMMER, MGC111107, RBP Associated Molecule 14-1, RAM14-1, Skeletal Muscle LIM Protein 1, SLIM1, SLIM-1, SLIM, XMPMA)

Anti -FHL1 (Four and a Half LIM Domains 1, FHL-1, FHL1A, FHL1B, FLH1A, bA535K18.1, KyoT,
LIM Protein SLIMMER, MGC111107, RBP Associated Molecule 14-1, RAM14-1, Skeletal Muscle LIM
Protein 1, SLIM1, SLIM-1, SLIM, XMPMA)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
FHL1 is a 36kD member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323aa in length. It contains one C4-type Zn-finger region (aa7-31), three distinct LIM domains (aa40-77; 101-158; 162-212) and a bipartite NLS (aa231-246). There is one 32kD splice variant that shows a 50 aa substitution for aa231-323. This creates a fourth LIM domain. There are also alternative start sites aa29-16 upstream of the standard site, a deletion of aa168-296, and an 11 aa substitution for aa81-91. Over aa93-166, human FHL1 is 97aa identical to mouse FHL1.
Catalog #F4149-77E1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 200ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa93-166 from human FHL1 expressed in E. coli (Q13642).
SpecificityRecognizes human FHL1. Species sequence homology: recombinant human FHL2 and rhFHL3 (1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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