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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, MGC111107, Skeletal Muscle LIM-protein 1, SLIM1, SLIM-1, SLIM, SLIMMER, XMPMA)

Anti -FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, FHL1A, FHL1B, FLH1A, KYOT,
MGC111107, Skeletal Muscle LIM-protein 1, SLIM1, SLIM-1, SLIM, SLIMMER, XMPMA)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
HL1, a LIM domain containing protein abundantly expressed by skeletal muscle, which has been linked with several muscular dystrophy-like muscle disorders, including Scapuloperoneal syndrome (SPS), X-linked severe early-onset reducing body myopathy (RBM), and X-linked myopathy with postural muscle atrophy (XMPMA).
Catalog #F4149-77G
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
ELISA: 1:32,000
Western Blot: 0.05-3ug/ml detects a band of ~30kD in human muscle cell lysates.
Immunohistochemistry (paraffin): 2-2.5ug/ml requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
Positive Control (Ihc)Human testis
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.05% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to CNKRFVFHNEQVY from the internal region of human FHL1 (NP_001440.1; NP_001153171.1).
SpecificityRecognizes human FHL1. Expected to recognize FHL1 isoforms 5 and 2, the latter being implicated as a negative regulator of RBP J- mediated transcription. Species sequence homology: canine, mouse, rat, bovine, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesFour and a Half LIM Domains Protein 1, FHL1 Protein, RP23-71G11.1


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