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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -FHL2 (Four and a Half LIM Domains 2, DRAL, SLIM3)

Anti -FHL2 (Four and a Half LIM Domains 2, DRAL, SLIM3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein–protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows
specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N- terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin.
Catalog #F4149-78E
ApplicationsSuitable for use in Immunofluorescence (paraformaldehyde fixed cells), Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence (paraformaldehyde fixed cells): 1:10
Western Blot: 1:10
Immunohistochemistry (frozen and paraffin): 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9G187
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified
ImmunogenBacterially expressed mouse GST-FHL2.
SpecificityRecognizes the LIM-only protein FHL2. F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. Species crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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